Effects of Dimethyl Sulfoxide in Cholesterol-Containing Lipid Membranes: A Comparative Study of Experiments In Silico and with Cells
نویسندگان
چکیده
Dimethyl sulfoxide (DMSO) has been known to enhance cell membrane permeability of drugs or DNA. Molecular dynamics (MD) simulations with single-component lipid bilayers predicted the existence of three regimes of action of DMSO: membrane loosening, pore formation and bilayer collapse. We show here that these modes of action are also reproduced in the presence of cholesterol in the bilayer, and we provide a description at the atomic detail of the DMSO-mediated process of pore formation in cholesterol-containing lipid membranes. We also successfully explore the applicability of DMSO to promote plasma membrane permeability to water, calcium ions (Ca(2+)) and Yo-Pro-1 iodide (Yo-Pro-1) in living cell membranes. The experimental results on cells in culture can be easily explained according to the three expected regimes: in the presence of low doses of DMSO, the membrane of the cells exhibits undulations but no permeability increase can be detected, while at intermediate DMSO concentrations cells are permeabilized to water and calcium but not to larger molecules as Yo-Pro-1. These two behaviors can be associated to the MD-predicted consequences of the effects of the DMSO at low and intermediate DMSO concentrations. At larger DMSO concentrations, permeabilization is larger, as even Yo-Pro-1 can enter the cells as predicted by the DMSO-induced membrane-destructuring effects described in the MD simulations.
منابع مشابه
The effect of Carbon nanotube on the most effective peptide in Alzheimer's disease in the presence of Dimethyl Sulfoxide: In Silico approach
Due to the non-polar nature of carbon nanotubes, their use in aqueous environments is limited. Therefore, auxiliary solvents such as dimethyl sulfoxide are used to study the interactions between the amyloid-β peptide and carbon nanotubes. In this work, the interaction of Aβ (1-42), the most effective peptide in the development of Alzheimer's disease, with the carbon nanotube was performed using...
متن کاملAssessment of Cytotoxicity of Dimethyl Sulfoxide in Human Hematopoietic Tumor Cell Lines
Background: Dimethyl Sulfoxide (DMSO) is a solvent most broadly used as a cryopreservative agent. Antitumor effects of DMSO is a recently recognized phenomenon. In this study, cytotoxic effects of DMSO on human monocytes and T leukemic cell lines has been investigated in vitro. Methods: Human leukemic T cells (Molt-4 and Jurkat) and monocytes (U937 and THP1) were cultured in complete RPMI medi...
متن کاملInfluence of Dimethyl Sulfoxide as a Penetration Enhancer of Piroxicam Gel Through Biological Skin
Piroxicam is a non-steroidal anti-inflammatory agent which has an extensive use in rheumatic disorders. Since its skin penetration is still a subject for research, the aim of this study was to evaluate the effect of dimethyl sulfoxide on percutaneous penetration of piroxicam gel formulation through skin. In this study, as a model, two types of 0.5% piroxicam new gels, so called red and gr...
متن کاملTransfer of amphotericin B from gel state vesicles to mycoplasma cells: biphasic action on potassium transport and permeability.
The action of amphotericin B on the K+ permeability of Mycoplasma mycoides var. capri cells, containing either cholesterol or ergosterol in their membranes, was studied. When the drug, solubilized in dimethyl sulfoxide, was added directly to the cell suspension, a slightly greater sensitivity to permeabilization was observed for ergosterol-containing cells, confirming the data reported in the l...
متن کاملAmeliorating effects of genestein: Study on mice liver glutathione and lipid peroxidation after irradiation
Background: Genistein is a soya isoflavone, which is found naturally in legumes, such as soybeans and chickpeas. Radiation–induced free radicals in turn impair the antioxidative defense mechanism, leading to an increased membrane lipid peroxidation that results in damage of the membrane bound enzyme and may lead to damage or death of cell. Hence, the lipid peroxidation is a good biomar...
متن کامل